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Florian Group

Stem Cell Aging

Understanding alterations of aged somatic stem cells and improve the regeneration of tissues

Aging is the first risk factor for most diseases and we believe that understanding the biology of stem cell aging will be critical to enhance stem cell function and preserve their regenerative capacity over time. Our mission is to end the threat of age-related diseases and promote longevity
M. Carolina Florian
ICREA Research Professor
Group Leader, Regenerative Medicine Program of IDIBELL
Group Leader of P-CMRC
mflorian@idibell.cat
Biosketch

Lab focus – Understanding epigenetic regulators of the aging process

Somatic stem cells have the ability to regenerate tissues over time. This capacity declines with age, disrupting tissue maintenance. Many age-related diseases, including cancer and sarcopenia, are likely consequence of stem cell dysfunction. Understanding how stem cells age and identifying intervention strategies to maintain stem cell function and regenerative capacity is thus likely to lead to new therapeutic approaches for maintaining our health as we age. Our lab uses mouse models to explore the biology of stem cell function and regeneration and to identify mechanisms of age-related stem cell dysfunction. Our focus is on epigenetic alterations that can be pharmacologically targeted and on the interactions between stem cells and the niche. Based on our findings we have identified intervention strategies to improve the regenerative capacity of aged stem cells and we have unravelled biological alterations in the stem cell niche that could help our understanding of disease development in the elderly.

Why it matters

Interventions that promote stem cell health are likely to provide new avenues to prolong human tissue health and to treat or prevent a wide range of age-related diseases. Coupling fundamental insight into stem cell biology to the exploration of such intervention strategies is likely to be a productive route for the identification of new and promising therapeutic approaches.

News

M Carolina Florian new ICREA Research Professor
ICREA, the Catalan Institution for Research and Advanced Studies, offers permanent, tenured positions to the most talented and extraordinary scientists and academics to come and work in Catalonia.

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P-CMR[C] researchers define a new mechanism of aging in a bone marrow stem cell in women
In women cells, the expression of chromosome X is silenced to balance gene expression between XX females and XY males.

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The team led by Dr. M. Carolina Florian at IDIBELL receives a European grant of 2 million euros to study bone marrow rejuvenation
With this project, Dr. M Carolina Florian, from the IDIBELL’s Regenerative Medicine Program, aims to improve the survival and quality of life of elderly patients undergoing chemotherapy

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Very proud to be one of the 2020 awardees!
Thanks to all the people working in my lab…

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State of ‘hibernation’ keeps haematopoietic stem cells young
Niches in the bone marrow protect haematopoietic stem cells from ageing

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Inhibition of Cdc42 activity extends murine lifespan
Cdc42 is a small RhoGTPase regulating multiple functions in eukaryotic cells…

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La inteligencia artificial, nueva aliada contra la leucemia
La leucemia mieloide aguda es un tipo de cáncer de la sangre muy agresivo. Afecta 10 veces……

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Job openings

Predoctoral Researcher to Join Dr. Florian’s Lab

Application submission deadline: 28/02/2022
The candidate will be involved in an international research team focusing on investigating regeneration of endothelial cells and vessels in the bone marrow. The team aims at developing and implementing cutting-edge technologies (3D-whole mount bone marrow microscopy analysis, single cell transplantation, single cell RNA-seq and ATAC-seq, deep learning-based multivariate data analyses) to identify how vessels regenerate in the bone marrow.
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Funding

Keywords

Stem Cells, Aging, Regenerative Medicine, Epigenetics, Chromatin, Hematopoietic Stem Cell, Bone Marrow Niche

Publications

Transplanting rejuvenated blood stem cells extends lifespan of aged immunocompromised mice. Montserrat-Vazquez S, Ali NJ, Matteini F, Lozano J, Zhaowei T, Mejia-Ramirez E, Marka G, Vollmer A, Soller K, Sacma M, Sakk V, Mularoni L, Mallm JP, Plass M, Zheng Y, Geiger H, Florian MC.NPJ Regen Med. 2022 Dec 29;7(1):78. doi: 10.1038/s41536-022-00275-y.
Aging of the Hematopoietic Stem Cell Niche: New Tools to Answer an Old Question. Matteini F, Mulaw MA, Florian MC.Front Immunol. 2021 Nov 11;12:738204. doi: 10.3389/fimmu.2021.738204. eCollection 2021.
Grigoryan A, Pospiech J, Krämer S, Lipka D, Liehr T, Geiger H, Kimura H, Mulaw MA, Florian MC. (2021) Attrition of X Chromosome Inactivation in Aged Hematopoietic Stem Cells. Stem Cell Reports 16(4):708-716.
Florian MC, Leins H, Gobs M, Han Y, Marka G, Soller K, Vollmer A, Sakk V, Nattamai KJ, Rayes A, Zhao X, Setchell K, Mulaw M, Wagner W, Zheng Y, Geiger H. (2020) Inhibition of Cdc42 activity extends lifespan and decreases circulatinginflammatory cytokines in aged female C57BL/6 mice. Aging Cell 19(9):e13208.
Mejia-Ramirez E, Florian MC. (2020) Understanding intrinsic hematopoietic stemcell aging. Haematologica 105(1):22-37.
Zjablovskaja P, Florian MC. (2019) Acute Myeloid Leukemia: Aging and Epigenetics. Cancers (Basel) 12(1):103.
Florian MC. L’estat d’’hibernació’ manté les cèl·lules mare hematopoètiques joves
Sacma M, Pospiech J, Bogeska R, de Back W, Mallm JP, Sakk V, Soller K, Marka G, Vollmer A, Karns R, Cabezas-Wallscheid N, Trumpp A, Mendez-Ferrer S, Milsom MD, Mulaw M, Geiger H, Florian MC (2019) Haematopoietic stem cells in perisinusoidal niches are protected from ageing. Nat Cell Biol in press
Grigoryan A, Guidi N, Senger K, Liehr T, Soller K, Marka G, Vollmer A, Markaki Y, Leonhardt H, Buske C, Lipka DB, Plass C, Zheng Y, Mulaw MA, Geiger H, Florian MC. (2018) LaminA/C regulates epigenetic and chromatin architecture changes upon aging of hematopoietic stem cells. Genome Biol 19(1):189.
Florian MC*, Sacma M, Klose M, Knudson L, Soller K, Marka G, Nattamai KJ, Sakk V, Zheng Y, Mulaw MA, Glauche I, Geiger H* (2018) Aging alters the epigenetic asymmetry of HSC division. DOI 10.1371/journal.pbio.2003389. PLOS Biology *corresponding authors
Florian MC, Klenk J, Marka G, Soller K, Kiryakos H, Peter R, Herbolsheimer F, Rothenbacher D, Denkinger M, Geiger H (2017) Expression and activity of the small RhoGTPase Cdc42 in blood cells of older adults are associated with age and cardiovascular disease. J Gerontol A Biol Sci Med Sci
Florian MC, Nattamai KJ, Dorr K, Marka G, Uberle B, Vas V, Eckl C, Andra I, Schiemann M, Oostendorp RA, Scharffetter-Kochanek K, Kestler HA, Zheng Y, Geiger H (2013) A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing. Nature 503: 392-6
Members
Current Members
Eva Mejía Ramírez de Arellano
Lab Manager
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Francesca Matteini
Post Doc
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Barbara Walter
PhD student
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Javier Lozano
Research Assistant
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Chang Sun
Joint-PhD student
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Pablo Iañez
Joint PhD student
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Sara Montserrat
Bioinformatics Technician
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Surya Cayre
Post Doc
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Rebecca Andersson
Post Doc
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Eleni Nikolopoulou
PhD student
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Alba Ferrer Perez
PhD student
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Laia Solé Castilla
PhD Student
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Dario Di Bari
Master student
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Eleni Nikolopoulou
PhD student
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Alba Ferrer Perez
PhD student
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Laia Solé Castilla
PhD Student
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Past Members
Ani Grigoryan
Johannes Pospiech
Noelle J. Ali
Polina Zjablovskaja
Francesco Maria Affuso
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M. Carolina Florian
Group Leader, Regenerative Medicine Program of IDIBELL
Group Leader of P-CMRC
mflorian@idibell.cat

Dr. M. Carolina Florian holds a PhD from the University of Milano (Italy). She pursued postdoctoral training at the Institute of Molecular Medicine in Ulm University (Germany) from 2009 to 2015. In 2016, she was awarded an Emmy Noether Grant from the German Research Foundation (DFG) dedicated to outstanding early-career researchers. This grant supported the establishment of her independent research team on Epigenetics of Stem Cell Aging. In 2018, she was appointed as group leader at CMRB (Spain).

Her research in the past 5 years strongly challenged the concept that aging is an irreversible process and showed that it is possible to pharmacologically target the aged-dependent alteration of stem cell epigenetic polarity and functionally rejuvenate aged HSCs in vivo. Somatic stem cells are central for tissue homeostasis and regeneration. Their age-dependent functional decline constitutes a hallmark of tissue attrition upon aging, eventually limiting health-span and lifespan. Dr. Florian’s work is committed to further grow the understanding of alterations affecting aged somatic stem cells and she is investigating changes of the epigenetic architecture that drive stem cell aging, to improve tissue attrition with age or even to prevent aging-associated diseases.

Eva Mejía Ramírez de Arellano
Lab Manager
emejia@idibell.cat

Dr. Eva Mejía obtained her Bachelor´s degree in Biochemistry at the Universidad Autónoma de Madrid in 2001. She performed her PhD studies in Molecular Biology at the Centro de Investigaciones Biológicas-CSIC in Madrid from 2002 to 2006, studying the molecular mechanisms of replication fork blockage in the yeast Schizosaccharomyces pombe under the direction of Dr. Pablo Hernández Valenzuela. She demonstrated the role of the protein Sap1 in replication fork blockage at the rDNA in S. pombe.

After that, Dr. Mejía moved to the USA to work as a Research Associate in Professor Paul Russell’s lab at the Scripps Research Institute in La Jolla, California from 2007 to 2011. She studied the genetics and the molecular mechanisms in S. pombe involved in Replication Checkpoint Maintenance and Double Strand Break Repair. Her results have contributed to the understanding of Brc1 role in Double-Strand Break (DSB) repair during S-phase, and also, the role of the complex Ku80-Ku70 in DSB repair through Homologous Recombination together with Ctp1.

In 2011 she moved back to Spain to work as a Postdoctoral Researcher at Professor Ferran Azorín’s lab where she studied the function of CENP-B homolog in S. pombe in its role in chromatin organization using ChIP-Seq.

Dr. Mejía joined the CMRB in April of 2013 as a Research Assistant.

Francesca Matteini
Post Doc
fmatteini@idibell.cat

Francesca Matteini obtained her bachelor degree in biological sciences in 2012 from the University of Florence. During this period she studied the effect of cosmic radiation on hereditary material after its exposure to extraterrestrial environment.

She followed her studies obtaining her master degree in cellular and molecular biology at the University of Florence in 2015. During her master’s internship she worked on the characterization of the autocrine and paracrine action of bone marrow-derived Mesenchymal Stromal Cells, focusing on role of sphingolipids. From October 2015 to February 2019 Francesca performed her PhD in human biology and medical genetics at the Sapienza University of Rome. During her PhD Francesca studied the role of the Poly(ADP-ribose)polymerase 1 (PARP1) in the transcriptional regulation of MyoD target genes.

Francesca joined the Center of Regenerative Medicine in Barcelona (CMRB) in March 2019 first with an Erasmus-Unipharma Graduates scholarship and then as postdoctoral researcher in Dr. Carolina Florian’s group, focusing on the role of the bone marrow niche in the regulation of HSCs function upon aging.

Barbara Walter
PhD Student
bwalter@idibell.cat

Barbara recently joined Carolina’s group as PhD student. She’s interested in epigenetics and alteration of the bone marrow microenvironment during acute myeloid leukaemia in the elderly. Focusing on finding serotype independent, protein-based vaccines against Streptococcus pneumoniae, she did her Bachelor in genetics at the University of Greifswald. She acquired her Master’s degree in molecular and cell biology at the Free University of Berlin. Her master thesis was focused on characterizing a novel Cre-DreERT2 mouse strain to get more insights into Multiple Myeloma.

Javier Lozano
Research Assistant
jlozano@idibell.cat

Dr. Javier Lozano obtained his Bachelor’s degree in biology at the Universidad Complutense de Madrid (UCM) in 2012. He collaborated at the Immunology Department of Medicine School of UCM, where he defended his Master thesis in the study of γδ T lymphocytes and Bowel Disease in mouse model Vcre XBP1flox TCRd-/- in 2013.

He performed his PhD at the Institut d’Investigació Sanitària Pere Virgili in Joan XXIII hospital of Tarragona from 2014 to 2018, identifying and characterising miRNAs as potential modulators of TNF-induced insulin resistance in obesity. He found and functionally analysed two microRNAs (miR-181a and miR-23a) altered by TNF-alfa action in differentiated post-insulin-stimulated adipocytes as well as in adipose tissue of obese patients. He also described miR-181a as a predictive serological biomarker for the risk of pre-diabetes. During this period he also supported other collaborative researches like the study of association and role of the cytokine TWEAK in non-alcoholic fatty liver disease, the study of human gut mycobiome in association to cardiovascular disease and the search for novel biomarkers for prostate cancer in blood, urine and semen. He also was granted with a fellow to make a 3 month stay at the Manchester Collaborative Centre for Inflammation Research (United Kingdom) in 2016. There, he supported research in regulation and function of the deubiquitinase USP12 in human macrophages.

Dr. Lozano redirected his professional career as research technician and joined to Experimental Sciences department of Universitat Pompeu Fabra (Barcelona) in 2018. There, he assumed the role as technician and laboratory manager for two years, covering laboratory needs of Cell Biology’s group focused in research in muscle stem cell regenerative biology and senescence in mouse models.

Dr. Lozano joined the Stem Cell Aging group at IDIBELL in April 2021 as research technician and lab manager.

Chang Sun
Joint-PhD student
csun@idibell.cat
Chang Sun completed her MSc of Cell Biology in Southwest University of China in 2021. During this time, she was working on the function and regulation mechanism of BmCasp8L gene in autophagy of insects and provided a theoretical basis for revealing the regulation mechanism of autophagy in metamorphosis.
After her graduation, she worked as an assistant researcher in Dr. Peng’s group in Institute of Systems and Physical Biology of Shenzhen Bay Laboratory. During this time, she was focus on the epigenetic regulation of H3K9me3 and Lamin A/C in CAR-T cell activation.
In 2023, she joined Dr. Florian’s group and became a predoctoral student in the Systems and Physical Biology Institute at SZBL and Stem Cell Aging group at IDIBELL where she will study regulation of epigenetics in HSC aging.

Pablo Iañez
Joint-PhD student
pablo.ianez@isglobal.org

Pablo holds a BSc in Biochemistry from the University of Granada and an MSc in Bioinformatics from the University of Copenhagen. He was awarded with a “Fundacion La Caixa” INPhINIT Incoming fellowship in 2022. He is interested in the potential application of machine learning and deep learning algorithms to biomedical data.

He has bioinformatics expertise in applying machine learning models in protein and genomic data, as well as working with single-cell data. He has participated in the ESPACE consortium inside the Human Cell Atlas, building the first multi-omics single-cell atlas of the human pancreas.

Now, he is a UPF predoctoral student in the Biomedical Data Science group at ISGlobal and Stem Cell Aging group at IDIBELL, where he will study the application of machine learning and computer vision algorithms to decipher the aging of stem cells using microscopy images.

Sara Montserrat Vázquez
Bioinformatics Technician
smontserrat@idibell.cat

Sara Montserrat Vázquez obtained her Bachelor’s degree in Genetics at Universitat Autònoma de Barcelona in 2015. She went on to study a Master’s degree in Cytogenetics and Reproductive Biology at the same university, where she analysed blood samples of patients with neurodevelopmental disorders using comparative genomic hybridization arrays as her master thesis. From 2017 to 2020 she worked as a cytogeneticist in laboratories of clinical analysis in Alicante and Barcelona. Her main duty was analysing karyotypes of different types of cells (from peripheral blood, bone marrow, amniotic fluid and miscarriage tissue), as well as performing fluorescence in situ hybridization analysis of spermatozoa.

To expand her knowledge, Sara studied a Master’s degree in Bioinformatics and Biostatistics at Universitat Oberta de Catalunya and she had the opportunity to do an internship at Mireya Plass’ group during the last trimester of 2020. She analysed published data from single-cell RNA sequencing experiments on brain samples and identified differentially expressed RNA binding protein genes between Alzheimer’s disease patients and controls.

Sara joined M. Carolina Florian’s group at P-CMRC on April 2021 as a bioinformatics technician.

Surya Cayre
Post Doc
scayre@idibell.cat

Dr Surya Cayre obtained her Bachelor’s degree in Cell biology and Pathophysiology at the Université Paul Sabatier (UPS) in Toulouse in 2014, and her Master’s degree in Pharmacology in 2016 (UPS, Toulouse). During her master internship, she focused her research on the characterization of the role of the recently-discovered hormone Elabela on the energy metabolism.

From January 2017 to June 2020, she performed her PhD in Cell Biology in the labs of Dr Bruno Goud and Dr Marina Glukhova at Institut Curie in Paris. She studied the role of the Golgiassociated RAB6 GTPase in the mouse mammary secretory lineage function during gestation and lactation, and found that RAB6 regulates the secretory differentiation and activation of the mammary gland by controlling STAT5 activation.

Surya joined P-CMRC in April 2021 as a postdoctoral researcher in Dr Carolina Florian’s group to work on the Vascular Endothelial Stem Cells in the regeneration of the bone marrow upon aging.

Rebecca Andersson
Post Doc

Rebecca Andersson started her career by studying Medical Biology at Linköping University in Sweden, where she got both her Bachelor’s and her Master’s degree in 2013. In her studies she focused on oxidative damage to extracellular enzymes in the human respiratory tract (bachelor studies) and worked with structural characterisation of the human ubiquitin-conjugating enzyme UBE2E1 (master project). She later joined the group of Prof. Thomas Nyström at the University of Gothenburg in Sweden, first as a project student working with the yeast peroxiredoxin Tsa1 and later as a PhD-student in 2016.

Rebecca’s PhD-work focused on protein quality control in ageing, and the way molecular chaperones support and even extend the normal replicative lifespan of the yeast Saccharomyces cerevisiae. Her work focused both on how the canonical molecular chaperones from the Hsp70-family maintain lifespan, but also how the non-canonical chaperone Tsa1 can protect the cell during oxidative stress and together with Hsp70 chaperones extend the lifespan of yeast. She also did work to characterise the novel nucleolar protein quality control compartment in yeast, which is the basis for proper protein damage segregation in the nucleus during cell division in yeast.

During her PhD, Rebecca developed a collaboration together with Dr. Florian to study protein quality control in haematopoietic stem cells and visited the lab at P-CMR[C] in 2019 with an EMBO Short Term Fellowship. After she earned her PhD-degree from the University of Gothenburg in the spring of 2021, she joined Carolina’s lab at P-CMR[C] as a postdoctoral researcher in October the same year. Here she will be studying the ageing human bone marrow microenvironment and stem cell niches.

Eleni Nikolopoulou
PhD student
einikolopoulou@idibell.cat

Eleni Nikolopoulou obtained her Bachelor’s degree in Biology, at the University of Patras in 2019. She carried out her Bachelor thesis in the department of Medicine, studying the role of key cell cycle regulators during mid-neurogenesis. In 2021, she completed her MSc in the Biomedical Sciences program (Medical School, University of Patras), specializing in the field of Stem Cells and Regenerative Medicine. During her Master thesis, she focused on the involvement of CDT1, a critical regulator of DNA replication, in colorectal carcinogenesis and specifically the stem-like properties of cancer cells.

Eleni joined Dr. Florian’s group at P-CMRC, first in November 2021 for a research internship under the framework of Erasmus+ and then in April 2022 as a predoctoral researcher. Her research work is focused on vascular endothelial stem cells and their role in the regeneration of the HSC niche upon aging.

Alba Ferrer Pérez
PhD student
aferrerp@idibell.cat

Alba Ferrer Pérez obtained her Bachelor’s degree in Biochemestry and Biomedical Science at Universitat de València in 2018. During the last year of university, she did two stayings at the University of Copenhagen and at the University of Virginia. From 2018 to 2020 she worked as a laboratory technician at Spanish National Center for Cardiovascular Research (CNIC) in Madrid, working on the characterization of the pathophysiology of atherosclerosis.

She acquired her Master’s degree in research in translational medicine at Universidad Complutense de Madrid in 2020. Her Master’s thesis was focused on characterizing the effects of TET2 mutation-driven clonal hematopoiesis on atherosclerosis regression.

She recently joined Carolina’s group at P-CMRC as PhD student, focusing on the role of the ageing bone marrow niche in leukemic cells.

Laia Solé Castilla
PhD student
lsole@idibell.cat
I obtained my Bachelor’s degree in Biology, at the University of Girona in 2022. During the last semester of my degree, January to June 2022, I did a staying at the University of Ghent in an Erasmus+ program to develop my Bachelor’s thesis in the department of Morphology, Medical Imaging, Orthopaedics, Physiotherapy, and Nutrition.
In June 2023, I obtained the Master of Clinical Research (with a specialty in Organ, Tissue and Cell Donation and Transplantation), at the University of Barcelona. In January 2023 I joined Florian’s lab to develop my Master’s thesis.
Past October 2023 I joined again Florian’s Lab as a predoctoral researcher. My research is focused on the epigenetic remodeling of HSC during aging.
Dario di Bari
Master student
ddibari@idibell.cat
Dario graduated with a Bachelor’s Degree in Biotechnology from Tor Vergata University (Rome-Italy), with a thesis centered on the effects of Autophagy on breast cancer regression.
Currently he is undertaking a Master’s Degree in Molecular and Cellular Biology at the University of Bologna (Bologna-Italy).
Since October 2023, Dario is carrying out an internship for his master’s thesis at IDIBELL, Hematopoietic Stem Cell Aging – Florian Lab. His project is focused on studying the epigenetic remodeling of murine ltHSC during aging.