hPSC generation and characterization

The B-SCB offers services of generation and characterization of hiPSC lines, including administrative support for the authorisation of projects involving the use and /or the generation of hiPSC. hiPSC can be generated from different cell types such as fibroblasts, keratinocytes, peripheral blood cells, umbilical cord blood and , tumour and urine cells among others. We offer integrative and non- integrative reprogramming methodologies including retrovirus, lentivirus, Sendai virus, episomal factors and mRNA techniques.

Characterization tests:

Alkaline phosphatase
Mycoplasma analysis
Integration and silencing test
Karyotype
Fingerprinting
Pluripotency marker expression (OCT2, SOX2, NANOG, SEA3, SSEA4, TRA1-60 y TRA1-81)
In vitro differentiation (two markers for germ layer)
In vivo differentiation (two markers for germ layer)

How to request hiPSC generation services

To request information about the hiPSC generation service, you can contact blc@idibell.cat .

Requests for the generation of cell lines must first be authorized by an Ethics Committee and Comisión de Garantías para la Donación y Utilización de Células y Tejidos Humanos del Instituto de Salud Carlos III.

Information regarding generation and sample donor details:

Request of Generation of IPSC I

Request of Generation of IPSC II

How to request hPSC characterization services

To request hPSC characterization services contact blc@idibell.cat.

List of services and fees

wdt_ID	Generation of iPSC lines fees (€)	Internal Public	External Public	Private
1	Original Cells
2	Derivation of primary culture (skin biopsy)	220	270	570
4	Expansion of frozen fibroblasts or keratinocytes	115	140	300
5	Cell isolation from blood sample	150	185	280
6	Cell isolation from cord blood cells	200	240	340
7	Cell isolation from urine sample (epithelial cells)	225	280	485
8	iPSC generation
9	iPSC generation/retrovirus	2330	2855	3970
10	iPSC generation/Sendai virus	3225	3950	5275
11	iPSC generation/episomal plasmids	2860	3500	4830

(21% IVA not included)

iPSC can be generated from different sources as fibroblasts, keratinocytes, blood, cord blood or even from epithelial cells from urine. Other cell types can be considered. We can generate iPSC lines using integrative (retrovirus and lentivirus) and non-integrative (Sendai virus, episomal plasmids or mRNA) methodologies.

The B-SCB also offers banking and registration of iPSC at the Spanish National Stem Cell Bank, as well as technical advice in administrative aspects.