Services: Generation, Characterization and Line Providement

Services

The B-SCB offers services of generation and characterization of iPSC lines, including administrative support for the authorisation of projects involving the use and /or the generation of iPSC. iPSC can be generated from different cell types such as fibroblasts, keratinocytes, peripheral blood cells, umbilical cord blood, some tumors and urine cells among others. We offer integrative and non- integrative reprogramming methodologies including retrovirus, lentivirus, Sendai virus, episomal factors and mRNA techniques.

Characterization tests:

Alkaline phosphatase
Mycoplasma analysis.
Integration and silencing test
Karyotype
Fingerprinting
Pluripotency marker expression (OCT2, SOX2, NANOG, SEA3, SSEA4, TRA1-60 y TRA1-81)
In vitro differentiation (two markers for germ layer)
In vivo differentiation (two markers for germ layer)

How to request iPSC generation services

To request information about the iPSC generation service, you can contact: blc@idibell.cat.
Information to be provided:

*Projects involving the generation and use of human induced pluripotent stem cells (hiPSCs) must be approved by an Ethics Committee. In Catalonia, the competent committee is the Comitè d’Ètica de la Recerca en Medicina Regenerativa de Catalunya (CERM). recerca.salut@gencat.cat.

*Projects involving generation and use of iPS for reproductive purposes, gamete production and interspecies primary chimera formation must be authorized by the “Comisión de Garantías para la Donación y Utilización de Células y Tejidos Humanos”.

How to request iPSC characterization services

Researchers may request complete or partial characterization of iPS lines generated in their centers.
To request iPSC characterization services contact: blc@idibell.cat .

How to request iPSC lines

Lines must be requested to BNLC.

https://www.isciii.es/servicios/biobancos/banco-nacional-lineas-celulares

Available iPSC lines

https://www.isciii.es/es/servicios/biobancos/banco-nacional-lineas-celulares/lineas-ips

How to request hESC lines

Lines must be requested to BNLC.

*Projects involving derivation and use of hESC must be previously authorized by an Ethics Committee and the Comisión de Garantías para la Donación y Utilización de Células y Tejidos Humanos. In Catalonia, the competent committee is the Comitè d’Ètica de la Recerca en Medicina Regenerativa de Catalunya (CERM). recerca.salut@gencat.cat.