Services: Generation, Characterization and Line Providement
Services
The B-SCB offers services of generation and characterization of iPSC lines, including administrative support for the authorisation of projects involving the use and /or the generation of iPSC. iPSC can be generated from different cell types such as fibroblasts, keratinocytes, peripheral blood cells, umbilical cord blood, some tumors and urine cells among others. We offer integrative and non- integrative reprogramming methodologies including retrovirus, lentivirus, Sendai virus, episomal factors and mRNA techniques.
Characterization tests:
- Alkaline phosphatase
- Mycoplasma analysis.
- Integration and silencing test
- Karyotype
- Fingerprinting
- Pluripotency marker expression (OCT2, SOX2, NANOG, SEA3, SSEA4, TRA1-60 y TRA1-81)
- In vitro differentiation (two markers for germ layer)
- In vivo differentiation (two markers for germ layer)
How to request iPSC generation services
Projects involving iPSC generation have to be approved by an Ethics Committee. *
To request information about the iPSC generation service, you can contact: blc@idibell.cat .
Information to be provided:
*Projects involving the generation and use of hiPS for reproductive purposes, gamete production and interspecies primary chimera formation must be authorized by the Comision de Garantías para la Donación y Utilización de Células y Tejidos Humanos”
How to request iPSC characterization services
To request iPSC characterization services contact: blc@idibell.cat .
How to request iPSC lines
Projects involving iPSC use have to be approved by an Ethics Committee. *
Lines must be requested to BNLC:
Available iPSC lines
*Projects involving generation and use of iPS for reproductive purposes, gamete production and interspecies primary chimera formation must be authorized by the Comision de Garantías para la Donación y Utilización de Células y Tejidos Humanos”
How to request hESC lines
Projects involving iPSC use have to be approved by an Ethics Committee. *
Projects involving derivation and use of hESC must be previously authorized by an Ethics Committee and the Comisión de Garantías para la Donación y Utilización de Células y Tejidos Humanos
Available hESC lines
List of services and fees
| wdt_ID | Generation of iPSC lines fees (€) | Internal Public | External Public | Private |
|---|---|---|---|---|
| 1 | Original Cells | |||
| 2 | Derivation of primary culture (skin biopsy) | 320 | 370 | 570 |
| 4 | Expansion of frozen fibroblasts or keratinocytes | 150 | 280 | 380 |
| 5 | Cell isolation from blood sample | 150 | 185 | 280 |
| 6 | Cell isolation from cord blood cells | 200 | 240 | 340 |
| 7 | Cell isolation from urine sample (epithelial cells) | 225 | 280 | 485 |
| 8 | iPSC generation | |||
| 9 | iPSC generation/retrovirus | 2330 | 2855 | 3970 |
| 10 | iPSC generation/Sendai virus | 3225 | 3950 | 5275 |
| 11 | iPSC generation/episomal plasmids | 2860 | 3500 | 4830 |
| 12 | iPSC generation/mRNA | 3500 | 4270 | 5500 |
| 13 | Characterization | |||
| 14 | Karyotype (x 2 clones) | 435 | 530 | 630 |
| 15 | Fingerprinting (original cells and iPSC) | 175 | 210 | 260 |
| 16 | Pluripotency | 395 | 485 | 565 |
| 17 | In vitro differentiation (Embryoid bodies) | 510 | 620 | 730 |
| 18 | In vivo differentiation (Teratomas) | 1075 | 1320 | 1350 |
| 19 | Banking | 600 | 740 | 860 |
| 20 | Registration at Banco Nacional de Líneas Celulares | 300 | 300 | 300 |
| 21 | Registered iPSC lines providement | 500 | 500 | 500 |
(21% IVA not included)
