hPSC generation and characterization
The B-SCB offers services of generation and characterization of hiPSC lines, including administrative support for the authorisation of projects involving the use and /or the generation of hiPSC. hiPSC can be generated from different cell types such as fibroblasts, keratinocytes, peripheral blood cells, umbilical cord blood and , tumour and urine cells among others. We offer integrative and non- integrative reprogramming methodologies including retrovirus, lentivirus, Sendai virus, episomal factors and mRNA techniques.
Characterization tests:
- Alkaline phosphatase
- Mycoplasma analysis
- Integration and silencing test
- Karyotype
- Fingerprinting
- Pluripotency marker expression (OCT2, SOX2, NANOG, SEA3, SSEA4, TRA1-60 y TRA1-81)
- In vitro differentiation (two markers for germ layer)
- In vivo differentiation (two markers for germ layer)
How to request hiPSC generation services
To request information about the hiPSC generation service, you can contact blc@idibell.cat .
Requests for the generation of cell lines must first be authorized by an Ethics Committee and Comisión de Garantías para la Donación y Utilización de Células y Tejidos Humanos del Insitiuto de Salud Carlos III.
Information regarding generation and sample donor details:
Request of Generation of IPSC I
Request of Generation of IPSC II
How to request hPSC characterization services
List of services and fees
iPSC can be generated from different sources as fibroblasts, keratinocytes, blood, cord blood or even from epithelial cells from urine. Other cell types can be considered. We can generate iPSC lines using integrative (retrovirus and lentivirus) and non-integrative (Sendai virus, episomal plasmids or mRNA) methodologies.
The B-SCB also offers banking and registration of iPSC at the Spanish National Stem Cell Bank, as well as technical advice in administrative aspects.
